APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:100 |
| 1:50 - 1:100 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant funsion protein containing a sequence corresponding to amino acids 1-200 of human PIK3C2A (NP_002636.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha , CPK , PI3-K-C2(ALPHA) , PI3-K-C2A , PI3K-C2-alpha , PI3K-C2alpha
Cellular Localization
Cell membrane,Golgi apparatus,Nucleus,Cytoplasm
Background
The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX65983 WB Image
WB analysis of various sample lysates using GTX65983 PI3 kinase C2 alpha antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX65983 IHC-P Image
IHC-P analysis of mouse heart tissue using GTX65983 PI3 kinase C2 alpha antibody.
Dilution : 1:100
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GTX65983 IHC-P Image
IHC-P analysis of rat heart tissue using GTX65983 PI3 kinase C2 alpha antibody.
Dilution : 1:100
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GTX65983 IP Image
IP analysis of HeLa cell lysate using GTX65983 PI3 kinase C2 alpha antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000
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GTX65983 IHC-P Image
IHC-P analysis of human liver damage tissue using GTX65983 PI3 kinase C2 alpha antibody.
Dilution : 1:100
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GTX65983 ICC/IF Image
ICC/IF analysis of C6 cells using GTX65983 PI3 kinase C2 alpha antibody.
Blue : DAPI
Dilution : 1:100
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REFERENCE
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REVIEW
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