GeneTex
United States (US)

PIAS1 antibody

GTX35181_20190131_IHC-P_19013102_572.jpg
GTX35181_20190131_WB_19013102_748.jpg

Cat No. GTX35181

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

72 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of PIAS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Inhibitor Of Activated Stat 1,Ddxbp1,Gbp,Gu/Rh-Ii,Zmiz3,Pias1

Cellular Localization

Nucleus,Nucleus speckle,PML body

Background

This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES
GTX35181_20190131_IHC-P_19013102_572.jpg

GTX35181 IHC-P Image

IHC-P analysis of formalin fixed human colon cancer tissue section using GTX35181 PIAS1 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX35181_20190131_WB_19013102_748.jpg

GTX35181 WB Image

WB analysis of HUVEC (A), Jurkat (B) whole cell lysates using GTX35181 PIAS1 antibody.

Package List Price ($)
$ 319