GeneTex
United States (US)

PIM1 (Active) recombinant protein

Cat No. GTX65203

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

316 nmol phosphate incorporated into the S6K substrate peptide per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).

Calculated MW

62.0 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Storage

Store at -80ºC. Product is stable for at least 6-12 months.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Expression System

Baculovirus (Sf9 insect cells)

Purification


Purity was assessed by SDS-PAGE (≥90%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PIM1, Serine/threonine-protein kinase Pim-1, Serine/threonineprotein kinase Pim1, Pim

Background

The proto-oncogene Pim1 belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Originally identified from Moloney murine leukemia virus induced T-cell lymphomas in mice, Pim1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others. Expression of Pim1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational. Accumulating data support that the expression of Pim1 is mediated through activation of the JAK/STAT pathway. Some of the substrates of Pim1 include p21 Cip1, nuclear mitotic appartus protein, PTP-U2S and Socs-1. Recently, Pim1 has been shown to enhance the activities of p100, c-Myb and Cdc 25a and in part this might explain reported effects of Pim1 on mitogenesis. Pim1 interacts with c-Myb via the DNA binding domain and regulates its transcriptional activity.

Research Area