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PKC beta (phospho Thr500) antibody

Orthogonal
Anti-PKC beta (phospho Thr500) antibody used in Western Blot (WB). GTX25817
Anti-PKC beta (phospho Thr500) antibody used in Western Blot (WB). GTX25817
Anti-PKC beta (phospho Thr500) antibody used in Western Blot (WB). GTX25817

Cat. No. GTX25817

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Rat
Package
50 μl ($399)
See all PKC beta 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Product Note

This antibody cross-reacts with PKC alpha [pT497] (88% homologous) and partially cross-reacts with PKC gamma [pT514] (63% homologous) and epsilon [pT566] (75% homologous), as determined by peptide competition experiments.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCbI and II that contains threonine 500.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C beta , PKC-beta , PKCB , PKCI(2) , PKCbeta , PRKCB1 , PRKCB2

Cellular Localization

Cytoplasm,Nucleus,Membrane

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PKC beta (phospho Thr500) antibody used in Western Blot (WB). GTX25817

GTX25817 WB Image

Orthogonal

WB analysis of K562 cells stimulated with PMA using GTX25817 PKC beta (phospho Thr500) antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal verifying that the antibody is phospho-specific.

Anti-PKC beta (phospho Thr500) antibody used in Western Blot (WB). GTX25817

GTX25817 WB Image

The data was published in the journal PLoS One in 2014. PMID: 24466133

Anti-PKC beta (phospho Thr500) antibody used in Western Blot (WB). GTX25817

GTX25817 WB Image

The data was published in the journal PLoS One in 2014. PMID: 24466133

REFERENCE

Application Reference

Guorong Lin et al. PLoS One 2014; 9 (1) : e86520 Complex regulation of PKCβ2 and PDK-1/AKT by ROCK2 in diabetic heart.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399