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PKC eta (phospho Thr655) antibody

Orthogonal
Anti-PKC eta (phospho Thr655) antibody used in Western Blot (WB). GTX25798

Cat. No. GTX25798

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human
Package
50 μl ($399)
See all PKC eta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
FACS 1:20
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Product Note

This antibody does not cross-react with any other PKC isoforms tested.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKC-eta that contains threonine 655.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C eta , PKC-L , PKCL , PRKCL , nPKC-eta

Cellular Localization

Cytoplasm

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-PKC eta (phospho Thr655) antibody used in Western Blot (WB). GTX25798

GTX25798 WB Image

Orthogonal

WB (peptide competition) analysis of Jurkat cells stimulated with PMA using GTX25798 PKC eta (phospho Thr655) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine containing peptide (Lane 3), the phosphopeptide immunogen (Lane 4), or the phosphopeptide corresponding to the immunogen from other PKC isoforms (Lane 5-9). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 10) eliminates the signal further verifying that the antibody is phospho-specific.
Dilution : 1:2000

REFERENCE

Application Reference

Lee PC et al. Am J Cancer Res 2023; 13 (10) : 4693-4707 Development of triazole-based PKC-inhibitors to overcome resistance to EGFR inhibitors in EGFR-mutant lung cancers.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399