APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , PC3 , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2 , MCF-7 , MDA-MB-231
Predict Reactivity
Zebrafish, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PLCB3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885296
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phospholipase C beta 3
Cellular Localization
Membrane; Peripheral membrane protein
Background
PLCB3 plays an important role in initiating receptor-mediated signal transduction. Activation of PLC takes place in many cells as a response to stimulation by hormones, growth factors, neurotransmitters, and other ligands (Lagercrantz et al., 1995 [PubMed 7607669]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX111100 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PLCB3 antibody (GTX111100) diluted at 1:1000 and competitor's antibody (sc-403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX111100 WB Image
phospholipase C beta 3 antibody detects phospholipase C beta 3 protein by western blot analysis. Various whole cell extracts of mouse and rat (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with phospholipase C beta 3 antibody (GTX111100) diluted by 1:1000.
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GTX111100 WB Image
phospholipase C beta 3 antibody detects phospholipase C beta 3 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with phospholipase C beta 3 antibody (GTX111100) diluted by 1:1000.
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GTX111100 WB Image
phospholipase C beta 3 antibody detects phospholipase C beta 3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with phospholipase C beta 3 antibody (GTX111100) diluted by 1:1000.
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REFERENCE
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REVIEW
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