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PLEKHA7 antibody

Anti-PLEKHA7 antibody used in Western Blot (WB). GTX131146
Anti-PLEKHA7 antibody used in IHC (Paraffin sections) (IHC-P). GTX131146
Anti-PLEKHA7 antibody used in Western Blot (WB). GTX131146

Cat. No. GTX131146

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PLEKHA7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , IMR32

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PLEKHA7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886430

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pleckstrin homology domain containing A7

Background

Required for zonula adherens biogenesis and maintenance. Acts via its interaction with KIAA1543/Nezha, which anchors microtubules at their minus-ends to zonula adherens, leading to recruit KIFC3 kinesin to junctional site.

Database

Research Area

DATA IMAGES

Anti-PLEKHA7 antibody used in Western Blot (WB). GTX131146

GTX131146 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PLEKHA7 antibody (GTX131146) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PLEKHA7 antibody used in IHC (Paraffin sections) (IHC-P). GTX131146

GTX131146 IHC-P Image

PLEKHA7 antibody detects PLEKHA7 protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded mouse colon.
Green: PLEKHA7 stained by PLEKHA7 antibody (GTX131146) diluted at 1:1000.
Red: E-Cadherin, stained byE-Cadherin antibody [GT477] (GTX629691) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PLEKHA7 antibody used in Western Blot (WB). GTX131146

GTX131146 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PLEKHA7 antibody (GTX131146) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Seungbeom Ko et al. J Lipid Atheroscler 2022; 11 (1) : 55-72
Application : WB
Reactivity : Mouse
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REVIEW

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Application Reference
Seungbeom Ko et al. J Lipid Atheroscler 2022; 11 (1):55-72
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169