APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Brain Tissue Slide(GTX22203) , Mouse Skeletal Muscle Tissue Lysate(GTX27938)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
PLEKHM3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human PLEKHM3.The immunogen is located within the last 50 amino acids of PLEKHM3.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
pleckstrin homology domain containing M3 , DAPR , PLEKHM1L
Cellular Localization
Cytoplasm,Golgi apparatus,Cell membrane
Background
PLEKHM3, also known as DAPR, is a member of the M family of Pleckstrin homology domain-containing proteins. PLEKHM3 was initially identified through chromatin immunoprecipitation and CpG microarray analysis examining proteins regulated by myocyte-enhancing factor 2. In C2C12 myoblast cells, PLEKHM3 binds to the PI3K signaling member protein kinase B in the cytosol prior to differentiation into myotubes. Following the initiation of differentiation, PLEKHM3 was also found in membrane fractions. Knockdown of PLEKHM3 expression by RNAi resulted in the inhibition of myotube formation, suggesting that PLEKHM3 is a key component required by myoblasts for orchestrating their differentiation during myogenesis.
Database
DATA IMAGES
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GTX85229 WB Image
WB analysis of mouse skeletal muscle tissue lysate using GTX85229 PLEKHM3 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX85229 IHC-P Image
IHC-P analysis of human brain tissue using GTX85229 PLEKHM3 antibody.
Working concentration : 5 μg/ml
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GTX85229 IHC-P Image
IHC-P analysis of human brain tissue using GTX85229 PLEKHM3 antibody.
Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
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