APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.1-0.3μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89972-PEP).
Product Note
This antibody is expected to recognise isoform 1 (NP_002660.1) and isoform 2 (NP_001188493.1).
Predict Reactivity
Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-PVSWKPEIIKRKRF, from the C Terminus of the protein sequence according to NP_002660.1; NP_001188493.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
pleiotropic regulator 1 , Cwc1 , PRL1 , PRP46 , PRPF46 , TANGO4
Cellular Localization
Nucleus , Nucleus speckle
Background
This gene encodes a core component of the cell division cycle 5-like (CDC5L) complex. The CDC5L complex is part of the spliceosome and is required for pre-mRNA splicing. The encoded protein plays a critical role in alternative splice site selection. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
Database
DATA IMAGES
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GTX89972 WB Image
WB analysis of HEK293 (A) and NIH-3T3 (B) nuclear lysates using GTX89972 PLRG1 antibody, C-term. Dilution : 0.1μg/ml Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for PLRG1 antibody, C-term (GTX89972). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PLRG1 antibody, C-term (GTX89972). Be the first to share your experience with this product.