PLRG1 antibody [C3], C-term

Cat. No. GTX106333

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all PLRG1 products

Datasheet
Document

View product citations for antibody GTX106333 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PLRG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241191

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pleiotropic regulator 1 , Cwc1 , PRL1 , PRP46 , PRPF46 , TANGO4

Cellular Localization

Nucleus speckle

Background

Necessary for spliceosome assembly and for pre-mRNA splicing.

Database

Gene ID: 5356 PLRG1
UniProt: O43660 PLRG1

DATA IMAGES

GTX106333 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX106333 diluted at 1:1000

GTX106333 ICC/IF Image

PLRG1 antibody [C3], C-term detects PLRG1 protein at nucleus by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PLRG1 protein stained by PLRG1 antibody [C3], C-term (GTX106333) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX106333 IHC-P Image

PLRG1 antibody [C3], C-term detects PLRG1 protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
PLRG1 antibody [C3], C-term (GTX106333) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty