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PML antibody

KO/KD
Anti-PML antibody used in IHC (Paraffin sections) (IHC-P). GTX55751
Anti-PML antibody used in Western Blot (WB). GTX55751
Anti-PML antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55751
Anti-PML antibody used in Western Blot (WB). GTX55751

Cat. No. GTX55751

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all PML products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human PML (NP_150253.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

promyelocytic leukemia , MYL , PP8675 , RNF71 , TRIM19

Cellular Localization

Nucleus,nucleoplasm,Cytoplasm,Endoplasmic reticulum membrane,Nucleolus

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PML antibody used in IHC (Paraffin sections) (IHC-P). GTX55751

GTX55751 IHC-P Image

IHC-P analysis of human appendix tissue using GTX55751 PML antibody.
Dilution : 1:100

Anti-PML antibody used in Western Blot (WB). GTX55751

GTX55751 WB Image

WB analysis of various sample lysates using GTX55751 PML antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PML antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55751

GTX55751 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX55751 PML antibody.
Blue : DAPI

Anti-PML antibody used in Western Blot (WB). GTX55751

GTX55751 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX55751 PML antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399