PODXL antibody [5F5]

Cat. No. GTX60437

Host	Mouse
Clonality	Monoclonal
Clone Name	5F5
Isotype	IgG1
Application	WB, IHC-P, FACS, ELISA
Reactivity	Human
Package	100 μl ($409)

See all PODXL products

Datasheet
Document

View product citations for antibody GTX60437 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PODXL expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

podocalyxin like , Gp200 , PC , PCLP , PCLP-1

Cellular Localization

Apical cell membrane,Membrane

Background

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]

Database

Gene ID: 5420 PODXL
UniProt: O00592 PODXL

Research Area

DATA IMAGES

GTX60437 ELISA Image

ELISA analysis of antigen using GTX60437 PODXL antibody [5F5].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

GTX60437 FACS Image

FACS analysis of HeLa cells using GTX60437 PODXL antibody [5F5].
Green : PODXL
Purple : negative control

GTX60437 WB Image

WB analysis of human PODXL (AA: 109-324) recombinant protein using GTX60437 PODXL antibody [5F5].

GTX60437 IHC-P Image

IHC-P analysis of lung cancer tissue (left) and kidney tissue (right) using GTX60437 PODXL antibody [5F5].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 409

Qty