APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.03-0.1μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89587-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-KYFAQEALTVLSLA, from the C Terminus of the protein sequence according to NP_055040.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein phosphatase 2 scaffold subunit Aalpha , MRD36 , PP2A-Aalpha , PP2AA , PP2AAALPHA , PR65A
Cellular Localization
Cytoplasm , Cell projection, dendrite
Background
This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
Database
Research Area
DATA IMAGES
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GTX89587 WB Image
WB analysis of human thymus lysate using GTX89587 PPP2R1A antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89587 IHC-P Image
IHC-P analysis of human heart using GTX89587 PPP2R1A antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX89587 IHC-P Image
IHC-P analysis of human thyroid Gland using GTX89587 PPP2R1A antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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REFERENCE
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REVIEW
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