APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-2μg/ml |
| 5-10μg/ml |
|
Note :
IHC-P
Human Heart shows textured cytoplasm staining in cardiomyocytes.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89777-PEP).
Predict Reactivity
Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-AYNMHSILSNTSAE, from the C Terminus of the protein sequence according to NP_006234.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein phosphatase 2 regulatory subunit B'alpha , B56A , B56alpha , PR61A
Cellular Localization
Cytoplasm , Nucleus
Background
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily. Alternative transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
Database
Research Area
DATA IMAGES
|
GTX89777 IHC-P Image
IHC-P analysis of human heart using GTX89777 PPP2R5A antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
|
|
|
GTX89777 WB Image
WB analysis of human muscle lysate using GTX89777 PPP2R5A antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for PPP2R5A antibody, C-term (GTX89777). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PPP2R5A antibody, C-term (GTX89777). Be the first to share your experience with this product.
