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PPP4C antibody

KO/KD
Anti-PPP4C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114659
Anti-PPP4C antibody used in Western Blot (WB). GTX114659
Anti-PPP4C antibody used in Western Blot (WB). GTX114659
Anti-PPP4C antibody used in Western Blot (WB). GTX114659

Cat. No. GTX114659

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PPP4C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , HepG2

Predict Reactivity

Rat, Rabbit, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPP4C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620612

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 4 catalytic subunit , PP-X , PP4 , PP4C , PPH3 , PPP4 , PPX

Cellular Localization

Cytoplasm , Nucleus , Centrosome

Background

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase.

Database

Research Area

DATA IMAGES

Anti-PPP4C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114659

GTX114659 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PPP4C(GTX114659) antibody at 1:500 dilution.

Anti-PPP4C antibody used in Western Blot (WB). GTX114659

GTX114659 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX114659 diluted at 1:500

Anti-PPP4C antibody used in Western Blot (WB). GTX114659

GTX114659 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX114659 diluted at 1:500

Anti-PPP4C antibody used in Western Blot (WB). GTX114659

GTX114659 WB Image

KO/KD

The data was published in the journal Cells in 2019. PMID: 31619012

REFERENCE

Application Reference

Burdova K et al. Cells 2019; 8 (10) WIP1 Promotes Homologous Recombination and Modulates Sensitivity to PARP Inhibitors.
Application : WB
Reactivity : Human
Jaiswal H et al. EMBO J 2017; (Epub) ATM/Wip1 activities at chromatin control Plk1 re-activation to determine G2 checkpoint duration.
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169