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PPT1 antibody [N1C3]

Anti-PPT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110677
Anti-PPT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110677
Anti-PPT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110677
Anti-PPT1 antibody [N1C3] used in Western Blot (WB). GTX110677
Anti-PPT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110677

Cat. No. GTX110677

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PPT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951370

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

palmitoyl-protein thioesterase 1 , CLN1 , INCL , PPT

Cellular Localization

Lysosome

Background

The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.

Database

Research Area

DATA IMAGES

Anti-PPT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110677

GTX110677 IHC-P Image

PPT1 antibody [N1C3] detects PPT1 protein at lysosome on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
PPT1 antibody [N1C3] (GTX110677) dilution: 1:500.
Scale bar = M μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PPT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110677

GTX110677 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using PPT1(GTX110677) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PPT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110677

GTX110677 IHC-P Image

PPT1 antibody [N1C3] detects PPT1 protein at lysosome on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
PPT1 antibody [N1C3] (GTX110677) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PPT1 antibody [N1C3] used in Western Blot (WB). GTX110677

GTX110677 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX110677 diluted at 1:3000

Anti-PPT1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110677

GTX110677 IHC-P Image

PPT1 antibody [N1C3] detects PPT1 protein at lysosome on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
PPT1 antibody [N1C3] (GTX110677) dilution: 1:500.
Scale bar = M_2 μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Erica L Gorenberg et al. PLoS Biol 2022; 20 (3) : e3001590 Identification of substrates of palmitoyl protein thioesterase 1?highlights roles of depalmitoylation in disulfide bond formation and synaptic function.
Liu WL et al. Molecules 2019; 22 (6) : pii: E929 Curcumin Inhibits LIN-28A through the Activation of miRNA-98 in the Lung Cancer Cell Line A549.
Application : WB
Reactivity : Human
You BJ et al. Chem Res Toxicol 2019; 32 (2) : 255-264
Application : WB
Reactivity : Human
Henderson MX et al. Acta Neuropathol 2015; Neuronal ceroid lipofuscinosis with DNAJC5/CSPα mutation has PPT1 pathology and exhibit aberrant protein palmitoylation.
Application : WB, ICC/IF, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169