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PRMT2 antibody

Anti-PRMT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103398
Anti-PRMT2 antibody used in Western Blot (WB). GTX103398

Cat. No. GTX103398

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PRMT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRMT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173526

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein arginine methyltransferase 2 , HRMT1L1

Background

Probably methylates the guanidino nitrogens of arginyl residues in some proteins. May play a role in transcriptional coactivation.

Database

Research Area

DATA IMAGES

Anti-PRMT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103398

GTX103398 ICC/IF Image

PRMT2 antibody detects PRMT2 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRMT2 protein stained by PRMT2 antibody (GTX103398) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-PRMT2 antibody used in Western Blot (WB). GTX103398

GTX103398 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRMT2 antibody (GTX103398) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169