GeneTex

  • Country / Location Selection

United States (US)

PRMT7 antibody

Anti-PRMT7 antibody used in Immunoprecipitation (IP). GTX116570
Anti-PRMT7 antibody used in IHC (Paraffin sections) (IHC-P). GTX116570
Anti-PRMT7 antibody used in IHC (Paraffin sections) (IHC-P). GTX116570
Anti-PRMT7 antibody used in ChIP assay (ChIP assay). GTX116570
Anti-PRMT7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116570
Anti-PRMT7 antibody used in IHC (Paraffin sections) (IHC-P). GTX116570

Cat. No. GTX116570

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PRMT7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRMT7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168451

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein arginine methyltransferase 7 , SBIDDS

Cellular Localization

Cytoplasm , cytosol , Nucleus

Background

Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PRMT7 antibody used in Immunoprecipitation (IP). GTX116570

GTX116570 IP Image

PRMT7 antibody immunoprecipitates PRMT7 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 μg of PRMT7 antibody (GTX116570) 7.5% SDS-PAGE The immunoprecipitated PRMT7 protein was detected by PRMT7 antibody (GTX116570) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PRMT7 antibody used in IHC (Paraffin sections) (IHC-P). GTX116570

GTX116570 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using PRMT7(GTX116570) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PRMT7 antibody used in IHC (Paraffin sections) (IHC-P). GTX116570

GTX116570 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using PRMT7(GTX116570) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PRMT7 antibody used in ChIP assay (ChIP assay). GTX116570

GTX116570 ChIP assay Image

Cross-linked ChIP was performed with NIH3T3 chromatin extract and 5 μg of either control rabbit IgG or anti-PRMT7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Apex2.
ChIP experiment and primer designs are based on J Biol Chem. 2012 Aug 24;287(35):29801-14.

Anti-PRMT7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116570

GTX116570 ICC/IF Image

PRMT7 antibody detects PRMT7 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRMT7 protein stained by PRMT7 antibody (GTX116570) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-PRMT7 antibody used in IHC (Paraffin sections) (IHC-P). GTX116570

GTX116570 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using PRMT7(GTX116570) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Jeong HJ et al. Cell Death Differ 2019; (Epub) Prmt7 promotes myoblast differentiation via methylation of p38MAPK on arginine residue 70.
Application : ChIP assay
Reactivity : Mouse
Jeong HJ et al. Diabetes 2016; (Epub) Prmt7 deficiency causes reduced skeletal muscle oxidative metabolism and age-related obesity.
Submit a Reference

REVIEW

There are currently no reviews for PRMT7 antibody (GTX116570). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169