APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.2-2.5 ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , HFF , Transfected 293T , U2OS
PROPERTIES
Form
Liquid
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a C-terminal region of Human PRMT8
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein arginine methyltransferase 8 , HRMT1L3 , HRMT1L4
Cellular Localization
Cell membrane
Background
Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).[supplied by OMIM, Mar 2008]
Database
Research Area
DATA IMAGES
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GTX47431 WB Image
WB analysis of tranfected 293T cells using GTX47431 PRMT8 antibody at 2.5μg/ml.
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GTX47431 WB Image
WB analysis of HeLa, HFF, and U2OS cells using GTX47431 PRMT8 antibody at 1:1000.Loading amount: 30ug.
Lanes:
1: HeLa cell lysate
2: HFF cell lysate
3: U2OS cell lysate
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REFERENCE
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REVIEW
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