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PRPS1 antibody

Anti-PRPS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102570
Anti-PRPS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102570
Anti-PRPS1 antibody used in Western Blot (WB). GTX102570

Cat. No. GTX102570

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PRPS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Drosophila, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRPS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037774

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoribosyl pyrophosphate synthetase 1 , ARTS , CMTX5 , DFN2 , DFNX1 , PPRibP , PRS-I , PRSI

Background

This gene encodes an enzyme that catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Defects in this gene are a cause of phosphoribosylpyrophosphate synthetase superactivity, Charcot-Marie-Tooth disease X-linked recessive type 5 and Arts Syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PRPS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102570

GTX102570 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PRPS1(GTX102570) antibody at 1:200 dilution.

Anti-PRPS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102570

GTX102570 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PRPS1(GTX102570) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PRPS1 antibody used in Western Blot (WB). GTX102570

GTX102570 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PRPS1 antibody (GTX102570) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169