GeneTex

  • Country / Location Selection

United States (US)

PRX II antibody

Anti-PRX II antibody used in Western Blot (WB). GTX100747
Anti-PRX II antibody used in IHC (Paraffin sections) (IHC-P). GTX100747
Anti-PRX II antibody used in IHC (Frozen sections) (IHC-Fr). GTX100747

Cat. No. GTX100747

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all PRX II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

A549 , HCT116

Predict Reactivity

Mouse, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRX II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10632187

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxiredoxin 2 , HEL-S-2a , NKEF-B , NKEFB , PRP , PRX2 , PRXII , PTX1 , TDPX1 , TPX1 , TSA

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The crystal structure of this protein has been resolved to 2.7 angstroms. Transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PRX II antibody used in Western Blot (WB). GTX100747

GTX100747 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PRX II antibody (GTX100747) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PRX II antibody used in IHC (Paraffin sections) (IHC-P). GTX100747

GTX100747 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Peroxiredoxin 2(GTX100747) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PRX II antibody used in IHC (Frozen sections) (IHC-Fr). GTX100747

GTX100747 IHC-Fr Image

Peroxiredoxin 2 antibody detects Peroxiredoxin 2 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Frozen section of rat liver.
Peroxiredoxin 2 antibody (GTX100747) diluted at 1:200.

REFERENCE

Application Reference

Huang HJ et al. Mol Biosyst 2014; (Epub) Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Application : IP
Reactivity : Human
Chilla A et al. Mol Cell Proteomics 2013; 12 (7) : 1926-38 Proteomic identification of VEGF-dependent protein enrichment to membrane caveolar-raft microdomains in endothelial progenitor cells.
Application : WB
Reactivity : Human
Lin ST et al. J Proteomics 2012; 75 (18) : 5822-47 proteomic analysis of proteins responsible for the development of * resistance in human uterine cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for PRX II antibody (GTX100747). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169