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PSMB10 antibody

Anti-PSMB10 antibody used in Western Blot (WB). GTX32818
Anti-PSMB10 antibody used in IHC (Paraffin sections) (IHC-P). GTX32818
Anti-PSMB10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32818
Anti-PSMB10 antibody used in IHC (Paraffin sections) (IHC-P). GTX32818

Cat. No. GTX32818

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PSMB10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human PSMB10 (NP_002792.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome subunit beta 10 , LMP10 , MECL1 , beta2i

Cellular Localization

Cytoplasm,Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PSMB10 antibody used in Western Blot (WB). GTX32818

GTX32818 WB Image

WB analysis of THP-1 cell lysate using GTX32818 PSMB10 antibody.
Dilution : 1:5000
Loading : 25μg per lane

Anti-PSMB10 antibody used in IHC (Paraffin sections) (IHC-P). GTX32818

GTX32818 IHC-P Image

IHC-P analysis of rat brain tissue using GTX32818 PSMB10 antibody.
Dilution : 1:200

Anti-PSMB10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32818

GTX32818 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32818 PSMB10 antibody.
Blue : DAPI

Anti-PSMB10 antibody used in IHC (Paraffin sections) (IHC-P). GTX32818

GTX32818 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32818 PSMB10 antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399