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PSMD10 antibody [3F6]

Overexpression
Anti-PSMD10 antibody [3F6] used in Western Blot (WB). GTX83773
Anti-PSMD10 antibody [3F6] used in Western Blot (WB). GTX83773
Anti-PSMD10 antibody [3F6] used in Flow cytometry (FACS). GTX83773
Anti-PSMD10 antibody [3F6] used in Flow cytometry (FACS). GTX83773

Cat. No. GTX83773

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3F6

Isotype

 IgG1

Application

 WB, FACS

Reactivity

 Human
Package
100 μl ($519)
See all PSMD10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
FACS 1:100
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PSMD10 (NP_002805) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome 26S subunit, non-ATPase 10 , dJ889N15.2 , p28 , p28(GANK)

Cellular Localization

Cytoplasm,Nucleus

Background

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and TP53/p53. Overexpression is leading to phosphorylation of RB1 and proteosomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to TP53/p53 and the mono- and polyubiquitination of TP53/p53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export.

Database

Research Area

DATA IMAGES

Anti-PSMD10 antibody [3F6] used in Western Blot (WB). GTX83773

GTX83773 WB Image

WB analysis of various cell lines using GTX83773 PSMD10 antibody [3F6].
Loading : 35 ug per lane

Anti-PSMD10 antibody [3F6] used in Western Blot (WB). GTX83773

GTX83773 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PSMD10 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83773 PSMD10 antibody [3F6].
Loading : 5 ug per lane

Anti-PSMD10 antibody [3F6] used in Flow cytometry (FACS). GTX83773

GTX83773 FACS Image

FACS analysis of HeLa cells using GTX83773 PSMD10 antibody [3F6].
Red : Primary antibody
Blue : Negative control antibody

Anti-PSMD10 antibody [3F6] used in Flow cytometry (FACS). GTX83773

GTX83773 FACS Image

FACS analysis of Jurkat cells using GTX83773 PSMD10 antibody [3F6].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519