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PTP1B antibody

KO/KD
Anti-PTP1B antibody used in Western Blot (WB). GTX55767
Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX55767
Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX55767
Anti-PTP1B antibody used in Western Blot (WB). GTX55767
Anti-PTP1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55767

Cat. No. GTX55767

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PTP1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 281-435 of human PTPN1 (NP_002818.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase non-receptor type 1 , PTP1B

Cellular Localization

Endoplasmic reticulum membrane

Background

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-PTP1B antibody used in Western Blot (WB). GTX55767

GTX55767 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX55767 PTP1B antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX55767

GTX55767 IHC-P Image

IHC-P analysis of rat brain tissue using GTX55767 PTP1B antibody.
Dilution : 1:100

Anti-PTP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX55767

GTX55767 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX55767 PTP1B antibody.
Dilution : 1:100

Anti-PTP1B antibody used in Western Blot (WB). GTX55767

GTX55767 WB Image

WB analysis of various sample lysates using GTX55767 PTP1B antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PTP1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55767

GTX55767 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX55767 PTP1B antibody.
Blue : DAPI

REFERENCE

Application Reference

Espinosa-Jim?nez T et al. Cell Biosci 2023; 13 (1) : 52 A novel rhein-huprine hybrid ameliorates disease-modifying properties in preclinical mice model of Alzheimer's disease exacerbated with high fat diet.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399