APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 4 μg/mL |
| 10 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Daudi Cell Lysate(GTX23951) , Human Small Intestine Tissue Lysate(GTX27923) , Human Small Intestine Tissue Slide
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Bit1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human Bit1.The immunogen is located within amino acids 80 - 130 of Bit1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
peptidyl-tRNA hydrolase 2 , BIT1 , CFAP37 , CGI-147 , IMNEPD , PTH , PTH 2 , PTH2
Cellular Localization
Mitochondrion
Background
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process (2). Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (3). It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE (2). Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (2).
Database
Research Area
DATA IMAGES
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GTX85497 IHC-P Image
IHC-P analysis of human small intestine tissue using GTX85497 PTRH2 antibody.
Working concentration : 20 μg/ml
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GTX85497 IHC-P Image
IHC-P analysis of human small intestine tissue using GTX85497 PTRH2 antibody.
Working concentration : 10 μg/ml
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GTX85497 WB Image
WB analysis of Daudi cell lysate using GTX85497 PTRH2 antibody.
Working concentration : (A) 1, (B) 2, and (C) 4 μg/ml
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REFERENCE
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REVIEW
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