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PTRH2 antibody

Anti-PTRH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85498
Anti-PTRH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85498
Anti-PTRH2 antibody used in Western Blot (WB). GTX85498

Cat. No. GTX85498

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

A20 Cell Lysate(GTX27180) , 3T3 Cell Lysate , 3T3/BALB Cell Slide

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Bit1 antibody was raised against a 15 amino acid synthetic peptide from near the amino-terminus of human Bit1.The immunogen is located within amino acids 20 - 70 of Bit1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidyl-tRNA hydrolase 2 , BIT1 , CFAP37 , CGI-147 , IMNEPD , PTH , PTH 2 , PTH2

Cellular Localization

Mitochondrion

Background

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process (2). Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (3). It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE (2). Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (2)

Database

Research Area

DATA IMAGES

Anti-PTRH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85498

GTX85498 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX85498 PTRH2 antibody.
Working concentration : 20 μg/ml

Anti-PTRH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85498

GTX85498 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX85498 PTRH2 antibody.
Dilution : 2 μg/ml

Anti-PTRH2 antibody used in Western Blot (WB). GTX85498

GTX85498 WB Image

WB analysis of A-20 cell lysate using GTX85498 PTRH2 antibody.
Dilution : 1μg/ml (Lane A), 2 μg/ml (Lane B), and 4μg/ml (Lane C)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399