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PUMA antibody [C2C3], C-term

Orthogonal
Anti-PUMA antibody [C2C3], C-term used in Western Blot (WB). GTX109675
Anti-PUMA antibody [C2C3], C-term used in Western Blot (WB). GTX109675
Anti-PUMA antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109675
Anti-PUMA antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX109675

Cat. No. GTX109675

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PUMA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Positive Control

HCT116 cells with 30uM cisplatin treatment for 24hr , K562 , THP-1

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PUMA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037804

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 binding component 3 , JFY-1 , JFY1 , PUMA

Cellular Localization

Mitochondrion

Background

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-PUMA antibody [C2C3], C-term used in Western Blot (WB). GTX109675

GTX109675 WB Image

Orthogonal

Sample (30 ug of whole cell lysate)
A: HCT116 cells with mock treatment for 0hr
B: HCT116 cells with 30uM cisplatin treatment for 24hr
15% SDS PAGE
GTX109675 diluted at 1:1000

Anti-PUMA antibody [C2C3], C-term used in Western Blot (WB). GTX109675

GTX109675 WB Image

PUMA antibody detects PUMA protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12 % SDS-PAGE, and blotted with PUMA antibody (GTX109675) diluted by 1:500

Anti-PUMA antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109675

GTX109675 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using PUMA(GTX109675) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PUMA antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX109675

GTX109675 IP Image

PUMA antibody [C2C3], C-term immunoprecipitates BCL2 binding component 3 protein in IP experiments.
IP samples: HCT-116 whole cell extract treat with 30uM cisplatin for 24 hr
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of BCL2 binding component 3 protein by 4 μg PUMA antibody [C2C3], C-term (GTX109675)
15 % SDS-PAGE
The immunoprecipitated BCL2 binding component 3 protein was detected by PUMA antibody [C2C3], C-term (GTX109675) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

Application Reference

Jingwen Li et al. RSC Adv 2019; 9 (67) : 39082-39089 New spirobisnaphthalenes from an endolichenic fungus strain CGMCC 3.15192 and their anticancer effects through the P53-P21 pathway.
Application : WB
Reactivity : Human
Wei RJ et al. Toxicol Appl Pharmacol 2016; (Epub) A microtubule inhibitor, ABT-751, induces autophagy and delays apoptosis in Huh-7 cells.
Application : WB
Reactivity : Human
Chen MF et al. BMC Cancer 2016; 16 : 532 Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.
Application : WB
Reactivity : Human
Chiu CF et al. PNAS 2016; 113 (18) : E2526-35 nf-κb-driven suppression of foxo3a contributes to egfr mutation-independent * resistance.
Application : WB
Reactivity : Human
Wu DW et al. Oncogene 2014; fhit loss confers * resistance in lung cancer via the akt/nf-κb/slug-mediated puma reduction.
Application : WB, IHC-P
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169