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PUS7 antibody [4C6]

KO/KD Overexpression
Anti-PUS7 antibody [4C6] used in Western Blot (WB). GTX83757
Anti-PUS7 antibody [4C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83757
Anti-PUS7 antibody [4C6] used in Western Blot (WB). GTX83757
Anti-PUS7 antibody [4C6] used in Flow cytometry (FACS). GTX83757
Anti-PUS7 antibody [4C6] used in Western Blot (WB). GTX83757
Anti-PUS7 antibody [4C6] used in Flow cytometry (FACS). GTX83757

Cat. No. GTX83757

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4C6

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all PUS7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PUS7 (NP_061915) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pseudouridine synthase 7

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-PUS7 antibody [4C6] used in Western Blot (WB). GTX83757

GTX83757 WB Image

WB analysis of various cell lines using GTX83757 PUS7 antibody [4C6].
Loading : 35 ug per lane

Anti-PUS7 antibody [4C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83757

GTX83757 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PUS7 plasmid using GTX83757 PUS7 antibody [4C6].

Anti-PUS7 antibody [4C6] used in Western Blot (WB). GTX83757

GTX83757 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PUS7 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83757 PUS7 antibody [4C6].
Loading : 5 ug per lane

Anti-PUS7 antibody [4C6] used in Flow cytometry (FACS). GTX83757

GTX83757 FACS Image

FACS analysis of Jurkat cells using GTX83757 PUS7 antibody [4C6].
Red : Primary antibody
Blue : Negative control antibody

Anti-PUS7 antibody [4C6] used in Western Blot (WB). GTX83757

GTX83757 WB Image

KO/KD

WB analysis of wild type and PUS7 knock out HeLa cell lysate(10μg per lane) using GTX83757 PUS7 antibody [4C6].
Dilution : 1:1000

Anti-PUS7 antibody [4C6] used in Flow cytometry (FACS). GTX83757

GTX83757 FACS Image

FACS analysis of HeLa cells using GTX83757 PUS7 antibody [4C6].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

Application Reference

Dan Song et al. J Exp Clin Cancer Res 2021; 40 (1) : 170 HSP90-dependent PUS7 overexpression facilitates the metastasis of colorectal cancer cells by regulating LASP1 abundance.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519