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PXK antibody [N2C2], Internal

Anti-PXK antibody [N2C2], Internal used in Western Blot (WB). GTX111746
Anti-PXK antibody [N2C2], Internal used in Western Blot (WB). GTX111746
Anti-PXK antibody [N2C2], Internal used in Western Blot (WB). GTX111746

Cat. No. GTX111746

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PXK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Mouse brain , K562 , THP-1 , HL-60 , U87-MG

Predict Reactivity

Rat, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PXK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951558

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PX domain containing serine/threonine kinase like , MONAKA , SLOB

Cellular Localization

Cytoplasm , Cell membrane; Peripheral membrane protein

Background

PXK binds to the Na,K-ATPase beta-1 (ATP1B1; MIM 182330) and beta-3 (ATP1B3; MIM 601867) subunits and modulates both Na,K-ATPase enzymatic and ion pump activities (Mao et al., 2005 [PubMed 16135750]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PXK antibody [N2C2], Internal used in Western Blot (WB). GTX111746

GTX111746 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111746 diluted at 1:1000

Anti-PXK antibody [N2C2], Internal used in Western Blot (WB). GTX111746

GTX111746 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PXK antibody [N2C2], Internal (GTX111746) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PXK antibody [N2C2], Internal used in Western Blot (WB). GTX111746

GTX111746 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PXK antibody [N2C2], Internal (GTX111746) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169