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PYGL antibody

Anti-PYGL antibody used in Immunoprecipitation (IP). GTX33448
Anti-PYGL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33448
Anti-PYGL antibody used in Western Blot (WB). GTX33448

Cat. No. GTX33448

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PYGL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PYGL (NP_002854.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887714

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen phosphorylase L , GSD6

Background

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-PYGL antibody used in Immunoprecipitation (IP). GTX33448

GTX33448 IP Image

IP analysis of HeLa cell lysate using GTX33448 PYGL antibody.
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

Anti-PYGL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33448

GTX33448 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX33448 PYGL antibody.
Blue : DAPI

Anti-PYGL antibody used in Western Blot (WB). GTX33448

GTX33448 WB Image

WB analysis of various sample lysates using GTX33448 PYGL antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Yarana C et al. Clin Cancer Res 2017; (Epub) extracellular vesicles released by cardiomyocytes in a *-induced cardiac injury mouse model contain protein biomarkers of early cardiac injury.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399