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PYGM antibody [C1C3]

Anti-PYGM antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110411
Anti-PYGM antibody [C1C3] used in Western Blot (WB). GTX110411
Anti-PYGM antibody [C1C3] used in Western Blot (WB). GTX110411
Anti-PYGM antibody [C1C3] used in Western Blot (WB). GTX110411
Anti-PYGM antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110411
Anti-PYGM antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110411

Cat. No. GTX110411

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all PYGM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

HeLa , Mouse brain

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PYGM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729460

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen phosphorylase, muscle associated

Background

Glycogen phosphorylase (EC 2.4.1.1) catalyzes and regulates the breakdown of glycogen to glucose-1-phosphate.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PYGM antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110411

GTX110411 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using PYGM antibody [C1C3] (GTX110411) at 1:300 dilution.

Anti-PYGM antibody [C1C3] used in Western Blot (WB). GTX110411

GTX110411 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
GTX110411 diluted at 1:1000

Anti-PYGM antibody [C1C3] used in Western Blot (WB). GTX110411

GTX110411 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYGM antibody [C1C3] (GTX110411) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PYGM antibody [C1C3] used in Western Blot (WB). GTX110411

GTX110411 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYGM antibody [C1C3] (GTX110411) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PYGM antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110411

GTX110411 IHC-P Image

PYGM antibody [C1C3] detects PYGM protein at cytoplasm on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
PYGM antibody [C1C3] (GTX110411) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PYGM antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110411

GTX110411 IHC-P Image

PYGM antibody [C1C3] detects PYGM protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
PYGM stained by PYGM antibody [C1C3] (GTX110411) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169