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Paxillin antibody

KO/KD Overexpression
Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Immunoprecipitation (IP). GTX125891
Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891

Cat. No. GTX125891

Reference 11
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Paxillin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

GFP-human Paxillin-transfected 293T cells , rat brain , HeLa

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Paxillin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885610

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

Database

Research Area

DATA IMAGES

Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891

GTX125891 ICC/IF Image

Paxillin antibody detects Paxillin protein at cytoskeleton by immunofluorescent analysis.
Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin protein stained by Paxillin antibody (GTX125891) diluted at 1:100.
Red: phalloidin staining.
Courtesy of a researcher who prefers to be anonymous.

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

KO/KD

Wild-type (WT) and Paxillin knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Immunoprecipitation (IP). GTX125891

GTX125891 IP Image

Paxillin antibody immunoprecipitates Paxillin protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 2 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Paxillin protein by 2 μg Paxillin antibody (GTX125891)
7.5 % SDS-PAGE
The immunoprecipitated Paxillin protein was detected by Paxillin antibody (GTX125891) diluted at 1:10000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891

GTX125891 IHC-P Image

Paxillin antibody detects Paxillin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Paxillin stained by Paxillin antibody (GTX125891) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

Overexpression

Paxillin antibody detects Paxillin protein by western blot analysis.
A. 1 μg 293T whole cell extract
B. 1 μg whole cell extract of GFP-human Paxillin-transfected 293T cells
7.5% SDS-PAGE
Paxillin antibody (GTX125891) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891

GTX125891 IHC-P Image

Paxillin antibody detects Paxillin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Paxillin stained by Paxillin antibody (GTX125891) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891

GTX125891 ICC/IF Image

The data was published in the journal J Cell Biol in 2020.PMID: 31816055

REFERENCE

Application Reference

Ramella M et al. iScience 2024; 27 (4) : 109440 Dual specificity kinase DYRK3 regulates cell migration by influencing the stability of protrusions.
Kumari R et al. Nat Commun 2024; 15 (1) : 2547 Focal adhesions contain three specialized actin nanoscale layers.
Ripamonti M et al. Commun Biol 2022; 5 (1) : 1025 A functional interaction between liprin-α1 and B56γ regulatory subunit of protein phosphatase 2A supports tumor cell motility.
Yin-Quan Chen et al. Front Cell Dev Biol 2022; 10 : 809738 Snail Augments Nuclear Deformability to Promote Lymph Node Metastasis of Head and Neck Squamous Cell Carcinoma.
Application : ICC/IF
Reactivity : Human
Schiweck J et al. Nat Commun 2021; 12 (1) : 1490 Drebrin controls scar formation and astrocyte reactivity upon traumatic brain injury by regulating membrane trafficking.
Application : ICC/IF
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

Paxillin antibody Cat. No. GTX125891

Rating
( Average 5 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 2 users reviews)
Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891
Date : Anonymous submitted on 30-Mar-2016
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Y1 cells
Blocking : Commercial Blocking buffer, 25°C, 1Hr
Fixation : paraformaldehyde, 25°C, 0.3Hr
Permeabilization /Antigen retrieval : 0.5% Triton X-100, 25°C, 10minHr
Primary Antibody : 1:500, 4°C, 16Hr
Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891
Date : Anonymous submitted on 20-Mar-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : MDA-MB-231 cells
Blocking : 3% BSA / 0.02% Triton-X100 in PBS, 25°C, 1Hr
Fixation : 4% paraformaldehyde
Primary Antibody : 1:100, 4°C, 16Hr
Submit a Review
Application Reference
Ramella M et al. iScience 2024; 27 (4):109440 Dual specificity kinase DYRK3 regulates cell migration by influencing the stability of protrusions.
Kumari R et al. Nat Commun 2024; 15 (1):2547 Focal adhesions contain three specialized actin nanoscale layers.
Ripamonti M et al. Commun Biol 2022; 5 (1):1025 A functional interaction between liprin-α1 and B56γ regulatory subunit of protein phosphatase 2A supports tumor cell motility.
Yin-Quan Chen et al. Front Cell Dev Biol 2022; 10:809738 Snail Augments Nuclear Deformability to Promote Lymph Node Metastasis of Head and Neck Squamous Cell Carcinoma.
Application : ICC/IF
Reactivity : Human
Schiweck J et al. Nat Commun 2021; 12 (1):1490 Drebrin controls scar formation and astrocyte reactivity upon traumatic brain injury by regulating membrane trafficking.
Application : ICC/IF
Reactivity : Mouse
Lin JW et al. Cell Death Discov 2021; 7 (1):35 Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signals.
Application : WB
Reactivity : Mouse
Masi I et al. Cell Rep 2021; 34 (9):108800 Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK.
Application : WB
Reactivity : Human
Atherton P et al. J Cell Biol 2020; 219 (1) Relief of talin autoinhibition triggers a force-independent association with vinculin.
Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.
Application : WB
Reactivity : Human
Megan R. Chastney et al. bioRxiv 2020; (Epub) Brain insulin resistance triggers early onset Alzheimer disease in Down syndrome.
Application : ICC/IF
Reactivity : Mouse
Borgo C et al. Sci Rep 2017; 7:42409 Generation and quantitative proteomics analysis of CK2α/α'(-/-) cells.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169