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Pirin antibody

Anti-Pirin antibody used in IHC (Paraffin sections) (IHC-P). GTX113584
Anti-Pirin antibody used in Western Blot (WB). GTX113584
Anti-Pirin antibody used in IHC (Paraffin sections) (IHC-P). GTX113584
Anti-Pirin antibody used in Western Blot (WB). GTX113584
Anti-Pirin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113584
Anti-Pirin antibody used in IHC (Paraffin sections) (IHC-P). GTX113584

Cat. No. GTX113584

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Pirin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HeLa , mouse liver

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pirin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617526

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pirin

Cellular Localization

Nucleus

Background

This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Pirin antibody used in IHC (Paraffin sections) (IHC-P). GTX113584

GTX113584 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MPG xenograft, using Pirin(GTX113584) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Pirin antibody used in Western Blot (WB). GTX113584

GTX113584 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
12% SDS PAGE
Pirin antibody
GTX113584 diluted at 1:1000

Anti-Pirin antibody used in IHC (Paraffin sections) (IHC-P). GTX113584

GTX113584 IHC-P Image

Pirin antibody detects Pirin protein at cytosol and nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Pirin antibody (GTX113584) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Pirin antibody used in Western Blot (WB). GTX113584

GTX113584 WB Image

Pirin antibody detects PIR protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
12 % SDS-PAGE
Pirin antibody (GTX113584) dilution: 1:1000

Anti-Pirin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113584

GTX113584 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Pirin(GTX113584) antibody at 1:500 dilution.

Anti-Pirin antibody used in IHC (Paraffin sections) (IHC-P). GTX113584

GTX113584 IHC-P Image

Pirin antibody detects Pirin protein at cytosol and nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
Pirin antibody (GTX113584) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169