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Pygopus 2 antibody

Anti-Pygopus 2 antibody used in Western Blot (WB). GTX120215
Anti-Pygopus 2 antibody used in Western Blot (WB). GTX120215
Anti-Pygopus 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX120215
Anti-Pygopus 2 antibody used in Western Blot (WB). GTX120215

Cat. No. GTX120215

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Pygopus 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

NIH-3T3 , A549 , H1299 , HCT116 , Huh7

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse Pygopus 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618939

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pygopus 2 , 1190004M21Rik

Database

Research Area

DATA IMAGES

Anti-Pygopus 2 antibody used in Western Blot (WB). GTX120215

GTX120215 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with pygopus 2 antibody (GTX120215) diluted at 1:1000.

Anti-Pygopus 2 antibody used in Western Blot (WB). GTX120215

GTX120215 WB Image

Sample (30 ug of whole cell lysate)
A:NIH-3T3 10% SDS PAGE
GTX120215 diluted at 1:10000

Anti-Pygopus 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX120215

GTX120215 IHC-P Image

pygopus 2 antibody detects Pygo2 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded lung adenocarcinoma. pygopus 2 antibody (GTX120215) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Pygopus 2 antibody used in Western Blot (WB). GTX120215

GTX120215 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with pygopus 2 antibody (GTX120215) diluted at 1:5000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169