GeneTex
  • Country / Location Selection

United States (US)

QKI antibody [N2C3]

Anti-QKI antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115969
Anti-QKI antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115969
Anti-QKI antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115969
Anti-QKI antibody [N2C3] used in Western Blot (WB). GTX115969
Anti-QKI antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115969
Anti-QKI antibody [N2C3] used in Western Blot (WB). GTX115969

Cat No. GTX115969

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , rat brain

Predict Reactivity

Bovine, Cat, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human QKI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

QKI, KH domain containing RNA binding , Hqk , QK , QK1 , QK3 , hqkI

Cellular Localization

Nucleus , Cytoplasm

Background

QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998 [PubMed 9671495]). QKI proteins regulate RNA splicing, export of target RNAs from the nucleus, translation of proteins, and RNA stability (Lauriat et al., 2008 [PubMed 17918747]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-QKI antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115969

GTX115969 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using QKI (GTX115969) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-QKI antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115969

GTX115969 ICC/IF Image

QKI antibody [N2C3] detects QKI protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: QKI protein stained by QKI antibody [N2C3] (GTX115969) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-QKI antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115969

GTX115969 IHC-Fr Image

QKI antibody [N2C3] detects QKI protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: QKI protein stained by QKI antibody [N2C3] (GTX115969) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-QKI antibody [N2C3] used in Western Blot (WB). GTX115969

GTX115969 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX115969 diluted at 1:1000

Anti-QKI antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115969

GTX115969 IHC-Fr Image

QKI antibody [N2C3] detects QKI protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: QKI protein stained by QKI antibody [N2C3] (GTX115969) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-QKI antibody [N2C3] used in Western Blot (WB). GTX115969

GTX115969 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI antibody [N2C3] (GTX115969) diluted at 1:1000.

REFERENCE
There are currently no references for QKI antibody [N2C3] (GTX115969). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for QKI antibody [N2C3] (GTX115969). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169