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RASGRF2 antibody [C2C3], C-term

Anti-RASGRF2 antibody [C2C3], C-term used in Western Blot (WB). GTX108622

Cat. No. GTX108622

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RASGRF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037844

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ras protein specific guanine nucleotide releasing factor 2 , GRF2 , RAS-GRF2

Cellular Localization

Cytoplasm , Cell membrane; Peripheral membrane protein , Endoplasmic reticulum membrane; Peripheral membrane protein

Background

RAS (MIM 190020) GTPases cycle between an inactive GDP-bound state and an active GTP-bound state. Guanine-nucleotide exchange factors (GEFs), such as RASGRFs, stimulate the conversion of the GDP-bound form into the active form.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RASGRF2 antibody [C2C3], C-term used in Western Blot (WB). GTX108622

GTX108622 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Ras GRF2 antibody [C2C3], C-term (GTX108622) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for RASGRF2 antibody [C2C3], C-term (GTX108622). Be the first to share your publications with this product.

REVIEW

RASGRF2 antibody [C2C3], C-term Cat. No. GTX108622

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 26-Aug-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : Brain homogenate
Amount used : 10 μg
Blocking : 5% non-fat dried milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169