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RPRD1B antibody

Anti-RPRD1B antibody used in Western Blot (WB). GTX119969
Anti-RPRD1B antibody used in IHC (Paraffin sections) (IHC-P). GTX119969
Anti-RPRD1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119969
Anti-RPRD1B antibody used in IHC (Paraffin sections) (IHC-P). GTX119969
Anti-RPRD1B antibody used in IHC (Paraffin sections) (IHC-P). GTX119969

Cat. No. GTX119969

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RPRD1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPRD1B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177175

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulation of nuclear pre-mRNA domain containing 1B , C20orf77 , CREPT , K-H , Kub5-Hera , NET60 , dJ1057B20.2

Database

Research Area

DATA IMAGES

Anti-RPRD1B antibody used in Western Blot (WB). GTX119969

GTX119969 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: HeLa
C: HepG2
10% SDS PAGE
GTX119969 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPRD1B antibody used in IHC (Paraffin sections) (IHC-P). GTX119969

GTX119969 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using RPRD1B(GTX119969) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPRD1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119969

GTX119969 ICC/IF Image

RPRD1B antibody detects RPRD1B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPRD1B protein stained by RPRD1B antibody (GTX119969) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-RPRD1B antibody used in IHC (Paraffin sections) (IHC-P). GTX119969

GTX119969 IHC-P Image

RPRD1B antibody detects RPRD1B protein at cytoplasm and nucleus in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
RPRD1B antibody (GTX119969) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPRD1B antibody used in IHC (Paraffin sections) (IHC-P). GTX119969

GTX119969 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RPRD1B(GTX119969) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Wen N et al. J Int Med Res 2020; 48 (1) : 300060519895089 Overexpression of cell-cycle related and expression-elevated protein in tumor (CREPT) in malignant cervical cancer.
Application : WB, IHC-P
Reactivity : Human
Bai J et al. Oncol Lett 2019; 18 (4) : 3743-3753 MicroRNA-300 inhibits the growth of hepatocellular carcinoma cells by downregulating CREPT/Wnt/帣-catenin signaling.
Application : WB
Reactivity : Human
Jinping Bai et al. Oncology Letters 2019; (Epub) MicroRNA?300 inhibits the growth of hepatocellular carcinoma cells by downregulating CREPT/Wnt/β?catenin signaling.
Zheng G et al. Biochem Biophys Res Commun 2016; 480 (3) : 436-442 High expression of CREPT promotes tumor growth and is correlated with poor prognosis in colorectal cancer.
Application : WB, IHC-P
Reactivity : Human
Liu T et al. Am J Transl Res 2016; 8 (5) : 2097-113 Inhibiting CREPT reduces the proliferation and migration of non-small cell lung cancer cells by down-regulating cell cycle related protein.
Application : WB, ICC/IF, IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169