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RRM2 antibody [N1C1]

KO/KD Orthogonal
Anti-RRM2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103193
Anti-RRM2 antibody [N1C1] used in Western Blot (WB). GTX103193
Anti-RRM2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103193
Anti-RRM2 antibody [N1C1] used in Western Blot (WB). GTX103193
Anti-RRM2 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103193
Anti-RRM2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103193
Anti-RRM2 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103193
Anti-RRM2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103193
Anti-RRM2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103193
Anti-RRM2 antibody [N1C1] used in Western Blot (WB). GTX103193
Anti-RRM2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103193

Cat. No. GTX103193

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all RRM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RRM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037925

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribonucleotide reductase regulatory subunit M2 , R2 , RR2 , RR2M

Cellular Localization

Cytoplasm

Background

This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RRM2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103193

GTX103193 IHC-P Image

RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
RRM2 antibody [N1C1] (GTX103193) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RRM2 antibody [N1C1] used in Western Blot (WB). GTX103193

GTX103193 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [N1C1] (GTX103193) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-RRM2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103193

GTX103193 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using RRM2 antibody [N1C1] (GTX103193) at 1:300 dilution.

Anti-RRM2 antibody [N1C1] used in Western Blot (WB). GTX103193

GTX103193 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [N1C1] (GTX103193) diluted at 1:1000.

Anti-RRM2 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103193

GTX103193 ICC/IF Image

RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: RRM2 protein stained by RRM2 antibody [N1C1] (GTX103193) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-RRM2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103193

GTX103193 IHC-P Image

RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
RRM2 antibody [N1C1] (GTX103193) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RRM2 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103193

GTX103193 ICC/IF Image

RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RRM2 protein stained by RRM2 antibody [N1C1] (GTX103193) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-RRM2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103193

GTX103193 IHC-P Image

RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
RRM2 antibody [N1C1] (GTX103193) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RRM2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103193

GTX103193 IHC-P Image

RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
RRM2 antibody [N1C1] (GTX103193) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RRM2 antibody [N1C1] used in Western Blot (WB). GTX103193

GTX103193 WB Image

KO/KD

The data was published in the journal PLoS One in 2014. PMID: 24637958

Anti-RRM2 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103193

GTX103193 IHC-P Image

The data was published in the journal PLoS One in 2014. PMID: 24637958

REFERENCE

Application Reference

LH Giang et al. J Exp Clin Cancer Res 2023; 42 (1) : 346 Targeting of RRM2 suppresses DNA damage response and activates apoptosis in atypical teratoid rhabdoid tumor
Application : WB
Reactivity : Human
Mirella Baroni et al. Cancers (Basel) 2021; 13 (7) Musashi1 Contribution to Glioblastoma Development via Regulation of a Network of DNA Replication, Cell Cycle and Division Genes.
Application : WB
Reactivity : Human
Baroni M et al. Cells 2021; 11 (1) The RNA-Binding Protein Musashi1 Regulates a Network of Cell Cycle Genes in Group 4 Medulloblastoma.
Application : WB
Reactivity : Human
Mu?oz-Monta?o W et al. BMC Cancer 2021; 21 (1) : 892
Application : IHC-P
Reactivity : Human
Kollareddy M et al. Cells 2021; 10 (4) Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
LH Giang et al. J Exp Clin Cancer Res 2023; 42 (1):346 Targeting of RRM2 suppresses DNA damage response and activates apoptosis in atypical teratoid rhabdoid tumor
Application : WB
Reactivity : Human
Mirella Baroni et al. Cancers (Basel) 2021; 13 (7) Musashi1 Contribution to Glioblastoma Development via Regulation of a Network of DNA Replication, Cell Cycle and Division Genes.
Application : WB
Reactivity : Human
Baroni M et al. Cells 2021; 11 (1) The RNA-Binding Protein Musashi1 Regulates a Network of Cell Cycle Genes in Group 4 Medulloblastoma.
Application : WB
Reactivity : Human
Mu?oz-Monta?o W et al. BMC Cancer 2021; 21 (1):892
Application : IHC-P
Reactivity : Human
Kollareddy M et al. Cells 2021; 10 (4) Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line.
Application : WB
Reactivity : Human
Thege FI et al. Anal Biochem 2019; (Epub) anti-EGFR capture mitigates EMT- and chemoresistance-associated heterogeneity in a resistance-profiling CTC platform.
Application : WB
Reactivity : Human
Hsieh YY et al. Cell Death Discovery 2016; 2:16027 Repositioning of a cyclin-dependent kinase inhibitor GW8510 as a ribonucleotide reductase M2 inhibitor to treat human colorectal cancer.
Application : WB
Reactivity : Human
Su YF et al. PLoS One 2014; 9 (3):e91644 The expression of ribonucleotide reductase m2 in the carcinogenesis of uterine cervix and its relationship with clinicopathological characteristics and prognosis of cancer patients.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169