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RUNX2 antibody

Orthogonal
Anti-RUNX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX81326
Anti-RUNX2 antibody used in Flow cytometry (FACS). GTX81326
Anti-RUNX2 antibody used in Western Blot (WB). GTX81326
Anti-RUNX2 antibody used in Western Blot (WB). GTX81326

Cat. No. GTX81326

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all RUNX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 445-474 amino acids of human RUNX2.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

RRID

AB_11176917

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

runt related transcription factor 2 , AML3 , CBF-alpha-1 , CBFA1 , CCD , CCD1 , CLCD , OSF-2 , OSF2 , PEA2aA , PEBP2aA

Cellular Localization

Nucleus

Background

This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-RUNX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX81326

GTX81326 IHC-P Image

IHC-P analysis of human tonsil using GTX81326 RUNX2 antibody.

Anti-RUNX2 antibody used in Flow cytometry (FACS). GTX81326

GTX81326 FACS Image

FACS analysis of NCI-H460 cells using GTX81326 RUNX2 antibody.
Green : primary antibody
Blue : negative control

Anti-RUNX2 antibody used in Western Blot (WB). GTX81326

GTX81326 WB Image

WB analysis of CEM cell lysate (35ug/lane) using GTX81326 RUNX2 antibody.

Anti-RUNX2 antibody used in Western Blot (WB). GTX81326

GTX81326 WB Image

Orthogonal

The data was published in the 2022 in Pharmaceuticals (Basel). PMID: 35631320

REFERENCE

Application Reference

Arpan Chowdhury et al. Pharmaceuticals (Basel) 2022; 15 (5) Activation of Nrf2/HO-1 Antioxidant Pathway by Heme Attenuates Calcification of Human Lens Epithelial Cells.
Application : WB
Reactivity : Human
Balogh E et al. Biomedicines 2021; 9 (4) Heme-Mediated Activation of the Nrf2/HO-1 Axis Attenuates Calcification of Valve Interstitial Cells.
Application : WB
Reactivity : Human
Chen YW et al. Materials Science and Engineering: C 2018; 91 : 679-687 Osteogenic and angiogenic potentials of the cell-laden hydrogel/mussel-inspired calcium silicate complex hierarchical porous scaffold fabricated by 3D bioprinting.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399