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Rabbit Anti-Mouse IgG (Fc) antibody [RM104]

Rabbit Anti-Mouse IgG (Fc) antibody [RM104]. GTX20295
Rabbit Anti-Mouse IgG (Fc) antibody [RM104]. GTX20295
Rabbit Anti-Mouse IgG (Fc) antibody [RM104]. GTX20295

Cat. No. GTX20295

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 RM104

Isotype

 IgG

Application

 WB, FACS, IP, ELISA

Reactivity

 Mouse
Package
100 μg ($369)
See all Mouse IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1 μg/mL - 0.5 μg/mL
FACS Assay dependent
IP Assay dependent
ELISA 0.005 μg/mL - 0.2 μg/mL
Not tested in other applications.

Product Note

This antibody reacts to the Fc region of all mouse IgG subclasses. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG. The Fc region of RM104 has been engineered to eliminate Fc receptor binding.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse IgG

Target Isotype

IgG Fc

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Rabbit Anti-Mouse IgG (Fc) antibody [RM104]. GTX20295

GTX20295 ELISA Image

ELISA analysis of mouse immunoglobulins (50 ng/well) using GTX20295 Rabbit Anti-Mouse IgG (Fc) antibody [RM104]. No cross reactivity with IgM,IgA, IgE, human or rat IgG.
Dilution : 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM104 was used as the primary antibody.

Rabbit Anti-Mouse IgG (Fc) antibody [RM104]. GTX20295

GTX20295 WB Image

WB analysis of nonreduced(-) and reduced(+) mouse immunoglobulins (20ng/lane) using GTX20295 Rabbit Anti-Mouse IgG (Fc) antibody [RM104]. No cross reactivity with IgM, IgA, or IgE.
Dilution : 0.2μg/ml

Rabbit Anti-Mouse IgG (Fc) antibody [RM104]. GTX20295

GTX20295 ELISA Image

ELISA analysis of different amount of immunogen proteins using serial diluted GTX20295 Rabbit Anti-Mouse IgG (Fc) antibody [RM104].

REFERENCE

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REVIEW

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Package List Price ($)
$ 369