GeneTex
United States (US)

Rabbit IgG (DyLight680)

GTX30811_FACS_20181130_19011519_580.jpg
GTX30811_ICC_IF_20181130_4_19011519_537.jpg
GTX30811_ICC_IF_20181130_1_19011519_485.jpg
GTX30811_ICC_IF_20181130_2_19011519_270.jpg
GTX30811_WB_20181130_19011519_269.jpg
GTX30811_ICC_IF_20181130_3_19011519_458.jpg

Cat No. GTX30811

Host Goat
Clonality Polyclonal
Application WB, ICC/IF, IHC-Fr, FACS, IP
Reactivity Rabbit
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.2, 1% BSA, 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. Protect from light.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography.

Conjugation

DyLight680

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
GTX30811_FACS_20181130_19011519_580.jpg

GTX30811 FACS Image

FACS analysis of permeabilized, fixed NRK cells (blue histogram) using MEK1/MAP2K1 antibody and labeled with GTX30811 Rabbit IgG antibody (DyLight™680).
Blue : Primary antibody with GTX30811
Green : Isotype control
Black : unstained control cells
Red : no-primary-antibody control

GTX30811_ICC_IF_20181130_4_19011519_537.jpg

GTX30811 ICC/IF Image

ICC/IF analysis of 4% PFA fixed cultured primary cortical neurons using GFAP antibody and labeled with GTX76793 Rabbit IgG antibody (DyLight680).
Permeabilization : 0.1% Triton X-100 in HBSS for 10 minutes at room temperature

GTX30811_ICC_IF_20181130_1_19011519_485.jpg

GTX30811 ICC/IF Image

ICC/IF analysis of HeLa cells using alpha Tubulin Antibody and labeled with GTX30811 Rabbit IgG antibody (DyLight™680).
Red : Primary antibody labeled with GTX30811

GTX30811_ICC_IF_20181130_2_19011519_270.jpg

GTX30811 ICC/IF Image

ICC/IF analysis of HeLa cells using alpha Tubulin Antibody and labeled with GTX30811 Rabbit IgG antibody (DyLight™680).
Red : Primary antibody labeled with GTX30811

GTX30811_WB_20181130_19011519_269.jpg

GTX30811 WB Image

WB analysis of whole cell extracts (30 µg lysate) of A549 (Lane 1) and HepG2 (Lane 2) using CacyBP Antibody and labeled with GTX30811 Rabbit IgG antibody (DyLight™680).

GTX30811_ICC_IF_20181130_3_19011519_458.jpg

GTX30811 ICC/IF Image

ICC/IF analysis of mouse embryonic fibroblasts (MEFs) using Connexcin 43 antibody and labeled with GTX30811 Rabbit IgG antibody (DyLight™680).
Red : Primary antibody labeled with GTX30811
Green : Actin

Package List Price ($)
$ 269