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Goat Anti-Rabbit IgG (F(ab')2) antibody, F(ab')2 fragment, pre-adsorbed (HRP)

Goat Anti-Rabbit IgG (F(ab')2) antibody, F(ab')2 fragment, pre-adsorbed (HRP). GTX26112

Cat. No. GTX26112

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG F(ab')2

Application

 WB, ELISA, IHC

Reactivity

 Rabbit
Package
250 μg ($179)
See all Rabbit IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2,000 - 1:10,000
ELISA 1:100,000
IHC 1:1,000 - 1:5,000
Not tested in other applications.

Product Note

This antibody has been pre-adsorbed against Bovine, Horse, Human, Mouse, Rat and Sheep serum proteins to ensure minimal cross-reactivity. Cross-reactivity with other species, however, is possible.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl, 1% BSA

Preservative

0.01% Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Rabbit IgG F(ab')2 fragment

Target Isotype

IgG F(ab')2

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining.

DATA IMAGES

Goat Anti-Rabbit IgG (F(ab')2) antibody, F(ab')2 fragment, pre-adsorbed (HRP). GTX26112

GTX26112 WB Image

Western Blot of GTX26112 Sample: Rabbit IgG. Load: 100 ng per lane. Secondary antibody: GTX26112 at 1:1,000 for 60 min at RT.Predicted/Observed size: 28 kDa for Rabbit IgG.

REFERENCE

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REVIEW

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Package List Price ($)
$ 179