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Rad51 antibody

KO/KD
Anti-Rad51 antibody used in Western Blot (WB). GTX118249
Anti-Rad51 antibody used in Western Blot (WB). GTX118249
Anti-Rad51 antibody used in Immunoprecipitation (IP). GTX118249
Anti-Rad51 antibody used in IHC (Paraffin sections) (IHC-P). GTX118249
Anti-Rad51 antibody used in Western Blot (WB). GTX118249
Anti-Rad51 antibody used in IHC (Paraffin sections) (IHC-P). GTX118249
Anti-Rad51 antibody used in IHC (Frozen sections) (IHC-Fr). GTX118249

Cat. No. GTX118249

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Rad51 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Jurkat , PC-12 , Rat-2 , 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein corresponding to human Rad51

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624798

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD51 recombinase , BRCC5 , FANCR , HRAD51 , HsRad51 , HsT16930 , MRMV2 , RAD51A , RECA

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Rad51 antibody used in Western Blot (WB). GTX118249

GTX118249 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody (GTX118249) diluted at 1:1000.

Anti-Rad51 antibody used in Western Blot (WB). GTX118249

GTX118249 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
10% SDS PAGE
GTX118249 diluted at 1:1000

Anti-Rad51 antibody used in Immunoprecipitation (IP). GTX118249

GTX118249 IP Image

Rad51 antibody immunoprecipitates Rad51 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 40 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Rad51 protein by 4 μg Rad51 antibody (GTX118249)
5 % SDS-PAGE
The immunoprecipitated Rad51 protein was detected by Rad51 antibody (GTX118249) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-Rad51 antibody used in IHC (Paraffin sections) (IHC-P). GTX118249

GTX118249 IHC-P Image

Rad51 antibody detects Rad51 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Rad51 antibody (GTX118249) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rad51 antibody used in Western Blot (WB). GTX118249

GTX118249 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody (GTX118249) diluted at 1:500.

Anti-Rad51 antibody used in IHC (Paraffin sections) (IHC-P). GTX118249

GTX118249 IHC-P Image

Rad51 antibody detects Rad51 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Rad51 antibody (GTX118249) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rad51 antibody used in IHC (Frozen sections) (IHC-Fr). GTX118249

GTX118249 IHC-Fr Image

The data was published in the journal Oncotarget in 2018. PMID: 29416789

REFERENCE

Application Reference

Liang ML et al. Oncotarget 2018; 9 (2) : 2527-2542 Significance of cyclin D1 overexpression in progression and radio-resistance of pediatric ependymomas.
Liang ML et al. Oncotarget 2018; 9 : 2527-2542 Significance of cyclin D1 overexpression in progression and radio-resistance of pediatric ependymomas.
Application : IHC-P
Reactivity : Human
Awwad SW et al. EMBO Rep 2017; 18 (5) : 745-764 NELF-E is recruited to DNA double-strand break sites to promote transcriptional repression and repair.
Vequaud E et al. Breast Cancer Res Treat 2015; Survivin contributes to DNA repair by homologous recombination in breast cancer cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169