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RAS antibody

Overexpression
Anti-RAS antibody used in Western Blot (WB). GTX132480
Anti-RAS antibody used in Western Blot (WB). GTX132480
Anti-RAS antibody used in Western Blot (WB). GTX132480
Anti-RAS antibody used in Western Blot (WB). GTX132480
Anti-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX132480
Anti-RAS antibody used in Western Blot (WB). GTX132480
Anti-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX132480
Anti-RAS antibody used in Western Blot (WB). GTX132480

Cat. No. GTX132480

Reference 8
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Goat, Frog, Squirrel
Package
100 μl ($399),
25 μl ($169)
See all RAS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Predict Reactivity

Human (100%)| Mouse (100%)| Rat (100%)| Zebrafish (100%)| Bovine (100%)| Chicken (100%)| Japanese medaka (100%)| Xenopus laevis (100%)| Xenopus tropicalis (100%)| Rhesus Monkey (100%)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human KRAS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886657

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Ras antibody [N1N3] detects Ras protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ras antibody [N1N3] (GTX132480) diluted at a dilution of 1:1000.

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Ras antibody [N1N3] detects Ras protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ras antibody [N1N3] (GTX132480) diluted at a dilution of 1:500.

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX132480

GTX132480 IHC-P Image

Ras antibody detects Ras protein at cell membrane in rat urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded rat urinary bladder.
Ras antibody (GTX132480) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (GI: gastrointestinal)

Anti-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX132480

GTX132480 IHC-P Image

Ras antibody detects Ras protein at cytoplasm in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Ras antibody (GTX132480) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Cheng CA et al. Adv Sci (Weinh) 2024; 11 (3) : e2304926 Ultrasensitive and High-Resolution Protein Spatially Decoding Framework for Tumor Extracellular Vesicles.
Hu W et al. JCI Insight 2021; 6 (3) NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.
Application : WB
Reactivity : Mouse
Cui J et al. Genes (Basel) 2020; 11 (9) MiR-184 Combined with STC2 Promotes Endometrial Epithelial Cell Apoptosis in Dairy Goats via RAS/RAF/MEK/ERK Pathway.
Application : WB
Zhu C et al. Animals (Basel) 2020; 10 (8) CircRNA8220 Sponges MiR-8516 to Regulate Cell Viability and Milk Synthesis via Ras/MEK/ERK and PI3K/AKT/mTOR Pathways in Goat Mammary Epithelial Cells.
Application : WB
Reactivity : Goat
Liu X et al. J Cell Physiol 2020; (Epub) miR-34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ-8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways.
Show More +
Submit a Reference

REVIEW

RAS antibody Cat. No. GTX132480

Rating
( Average 4.5 based on 4 users reviews)
Application
Western Blot(WB)
( Average 4.3 based on 3 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-RAS antibody used in Western Blot (WB). GTX132480
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : MIAPaca2 cell lysates
Amount used : 10μg
Blocking : 2% BSA, 25°C, 1Hr
Primary Antibody :
Anti-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX132480
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : colon cancer tissue
Fixation : Citrate buffer, 0.3°C
Primary Antibody : 1:500, 4°C, 16Hr
Anti-RAS antibody used in Western Blot (WB). GTX132480
Date : Anonymous submitted on 17-Jun-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 60μg
Blocking : 5% milk, 24°C, 1.5Hr
Primary Antibody : 1:1200, 4°C, 16Hr
Anti-RAS antibody used in Western Blot (WB). GTX132480
Date : Anonymous submitted on 29-Apr-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC3 cells
Amount used : 30μg
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 24Hr
Submit a Review
Application Reference
Cheng CA et al. Adv Sci (Weinh) 2024; 11 (3):e2304926 Ultrasensitive and High-Resolution Protein Spatially Decoding Framework for Tumor Extracellular Vesicles.
Hu W et al. JCI Insight 2021; 6 (3) NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.
Application : WB
Reactivity : Mouse
Cui J et al. Genes (Basel) 2020; 11 (9) MiR-184 Combined with STC2 Promotes Endometrial Epithelial Cell Apoptosis in Dairy Goats via RAS/RAF/MEK/ERK Pathway.
Application : WB
Zhu C et al. Animals (Basel) 2020; 10 (8) CircRNA8220 Sponges MiR-8516 to Regulate Cell Viability and Milk Synthesis via Ras/MEK/ERK and PI3K/AKT/mTOR Pathways in Goat Mammary Epithelial Cells.
Application : WB
Reactivity : Goat
Liu X et al. J Cell Physiol 2020; (Epub) miR-34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ-8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways.
Zhang Y et al. Biochim Biophys Acta 2018; 1862 (8):1721-1728 FoxO4 activity is regulated by phosphorylation and the cellular environment during dehydration in the African clawed frog, Xenopus laevis.
Application : WB
Reactivity : Frog
Chen PH et al. Neuropharmacology 2016; (Epub) the chac1-inhibited notch3 pathway is involved in *-induced glioma cytotoxicity.
Application : WB
Reactivity : Human
Zhang Y et al. Cryobiology 2016; 73 (2):112-9 Inhibition of skeletal muscle atrophy during torpor in ground squirrels occurs through downregulation of MyoG and inactivation of Foxo4.
Application : WB
Reactivity : Squirrel
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169