GeneTex
United States (US)

Ras antibody

Overexpression
GTX132480_41717_20150427_WB_18121410_350.jpg
GTX132480_41717_20160616_WB_Z_18121410_885.jpg
GTX132480_41717_20150717_IHC-P_18121410_993.jpg
GTX132480_41717_20150717_IHC-P_R_18121410_722.jpg
GTX132480_41717_20150813_WB_B_18121410_551.jpg

Cat No. GTX132480

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat, Zebrafish, Frog, Squirrel
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

A549 , H1299 , HCT116 , KRAS , HRAS , NRAS , zebrafish liver , zebrafish GI , whole zebrafish ,
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human KRAS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX132480_41717_20150427_WB_18121410_350.jpg

GTX132480 WB Image

Ras antibody [N1N3] detects Ras protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ras antibody [N1N3] (GTX132480) diluted at a dilution of 1:1000.

GTX132480_41717_20160616_WB_Z_18121410_885.jpg

GTX132480 WB Image

Various tissue extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras antibody [N1N3] (GTX132480) diluted at 1:500. (GI: gastrointestinal)

GTX132480_41717_20150717_IHC-P_18121410_993.jpg

GTX132480 IHC-P Image

Ras antibody detects Ras protein at cytoplasm in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Ras antibody (GTX132480) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX132480_41717_20150717_IHC-P_R_18121410_722.jpg

GTX132480 IHC-P Image

Ras antibody detects Ras protein at cell membrane in rat urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded rat urinary bladder.
Ras antibody (GTX132480) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX132480_41717_20150813_WB_B_18121410_551.jpg

GTX132480 WB Image

Overexpression

Ras antibody detects Ras protein by western blot analysis. Non-transfected (-) and transfected (+) whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ras antibody (GTX132480) at a dilution of 1:5000.

REFERENCE
REVIEW

Ras antibody Cat No. GTX132480

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 17-Jun-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 60μg
Blocking : 5% milk, 24°C, 1.5Hr
Primary Antibody : 1:1200, 4°C, 16Hr
Date : Anonymous submitted on 29-Apr-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC3 cells
Amount used : 30μg
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 24Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf