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Rat EGF protein (active)

Rat EGF protein (active). GTX00373-pro
Rat EGF protein (active). GTX00373-pro
Rat EGF protein (active). GTX00373-pro

Cat No. GTX00373-pro

Application Functional Assay
Species Rat
Package
10 μg ($259)
APPLICATION

Application Note

Epidermal growth factor (EGF) binding to EGFR, results in cellular proliferation, differentiation, and survival. To test the effect of EGF on cell proliferation, 3T3 fibroblasts cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 1% serum standard DMEM  prior to the addition of various concentrations of recombinant rat EGF. After incubated for 96h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of 3T3 cells after incubation with EGF for 96h observed by inverted microscope.

Observed MW

38 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asn974~Arg1026 (NP_036974.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

epidermal growth factor

Background

binds the epidermal growth factor receptor; may play a role in MAP kinase mediated signaling pathways [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Rat EGF protein (active). GTX00373-pro

GTX00373-pro Functional Assay Image

Cell proliferation effect of GTX00373-pro Rat EGF protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated 3T3 cells cultured in DMEM for 96hrs.
(B) 3T3 cells cultured in DMEM, stimulated with 1 ng/ml EGF for 96hrs.

Rat EGF protein (active). GTX00373-pro

GTX00373-pro Image

SDS-PAGE analysis of GTX00373-pro Rat EGF protein (active).

Rat EGF protein (active). GTX00373-pro

GTX00373-pro Image

WB analysis of GTX00373-pro Rat EGF protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 259