RbAp48 antibody [11G10]

Cat. No. GTX70232

Reference 11

Host	Mouse
Clonality	Monoclonal
Clone Name	11G10
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, IP, ChIP assay, Functional Assay, Blocking, IHC
Reactivity	Human, Mouse
Package	100 μl ($399)

See all RbAp48 products

View product citations for antibody GTX70232 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	Assay dependent
IP	Assay dependent
ChIP assay	Assay dependent
Functional Assay	Assay dependent
Blocking	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

MOLT4 , HeLa , LB22 , SH-SY5Y , SH-SY5Y nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_372869

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB binding protein 4, chromatin remodeling factor , NURF55 , RBAP48 , lin-53

Cellular Localization

Nucleus

Background

This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Database

Gene ID: 5928 RBBP4
UniProt: Q09028 RBBP4

Research Area

DATA IMAGES

GTX70232 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] (GTX70232) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70232 ChIP assay Image

RbAp48 antibody immunoprecipitates RbAp48 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune mouse IgG B. 5 μg of RbAp48 antibody (GTX70232) The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.

GTX70232 IHC Image

GTX70232 WB Image

SH-SY5Y whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] (GTX70232) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.