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Renin Receptor antibody [N3C3]

Anti-Renin Receptor antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114169
Anti-Renin Receptor antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114169
Anti-Renin Receptor antibody [N3C3] used in Western Blot (WB). GTX114169
Anti-Renin Receptor antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114169
Anti-Renin Receptor antibody [N3C3] used in Western Blot (WB). GTX114169
Anti-Renin Receptor antibody [N3C3] used in Western Blot (WB). GTX114169
Anti-Renin Receptor antibody [N3C3] used in Western Blot (WB). GTX114169

Cat. No. GTX114169

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Renin Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036296

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase H+ transporting accessory protein 2 , APT6M8-9 , ATP6IP2 , ATP6M8-9 , ELDF10 , HT028 , M8-9 , MRXE , MRXSH , MSTP009 , PRR , RENR , XMRE , XPDS

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Renin Receptor antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114169

GTX114169 ICC/IF Image

Renin Receptor antibody [N3C3] detects Renin Receptor protein by immunofluorescent analysis.
Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Renin Receptor stained by Renin Receptor antibody [N3C3] (GTX114169) diluted at 1:500.
Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Renin Receptor antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114169

GTX114169 ICC/IF Image

Renin Receptor antibody detects Renin Receptor protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Renin Receptor stained by Renin Receptor antibody (GTX114169) diluted at 1:500.

Anti-Renin Receptor antibody [N3C3] used in Western Blot (WB). GTX114169

GTX114169 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Renin Receptor antibody [N3C3] (GTX114169) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Renin Receptor antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114169

GTX114169 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HepG2, using Renin Receptor (GTX114169) antibody at 1:200 dilution.

Anti-Renin Receptor antibody [N3C3] used in Western Blot (WB). GTX114169

GTX114169 WB Image

The data was published in the journal Oncotarget in 2019. PMID: 31452834

Anti-Renin Receptor antibody [N3C3] used in Western Blot (WB). GTX114169

GTX114169 WB Image

The data was published in the journal Oncotarget in 2019. PMID: 31452834

Anti-Renin Receptor antibody [N3C3] used in Western Blot (WB). GTX114169

GTX114169 WB Image

The data was published in the journal Oncotarget in 2019. PMID: 31452834

REFERENCE

REVIEW

Renin Receptor antibody [N3C3] Cat. No. GTX114169

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 30-Jun-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : Hela cells
Amount used : 19 μg
Blocking : 3% skim milk, 25°C, 1Hr
Primary Antibody : 1:2000, 1°C, 25Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169