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S Tag antibody [GT247]

Anti-S Tag antibody [GT247] used in Immunoprecipitation (IP). GTX628791
Anti-S Tag antibody [GT247] used in Western Blot (WB). GTX628791

Cat. No. GTX628791

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT247

Isotype

 IgG2b

Application

 WB, IP

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all S Tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IP 1:500-1:1000
Not tested in other applications.

Positive Control

S-tagged protein expressing 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to S Tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888053

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-S Tag antibody [GT247] used in Immunoprecipitation (IP). GTX628791

GTX628791 IP Image

S tag antibody immunoprecipitates S-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of S-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of S-fused protein by 2 μg of S tag antibody (GTX628791) 12% SDS-PAGE The immunoprecipitated S-fused protein was detected by S tag antibody (GTX628791) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-S Tag antibody [GT247] used in Western Blot (WB). GTX628791

GTX628791 WB Image

S Tag antibody detects S protein by western blot analysis.
A. 30 μg 293T whole cell lysate/eztract
B. 30 μg whole cell lysate/extract of S-tagged protein expressing 293T cells
12% SDS-PAGE
S Tag antibody (GTX628791) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Senese S et al. Mol Biol Cell 2015; 26 (3) : 440-52 A unique insertion in STARD9's motor domain regulates its stability.
Application : WB
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations