PRODUCT
Summary
S100B, a member of the S100 family of calcium-binding cytosolic proteins with a predicted molecular weight of 11kDa, is an independent prognostic marker for melanoma. Elevated S100B and lactate dehydrogenase (LDH) levels are clinically useful for the detection of tumor progression and metastasis.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
V5-human S100B-transfected 293T cells
Predict Reactivity
Rabbit, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.14 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human S100 beta Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886037
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
S100 calcium binding protein B , NEF , S100 , S100-B , S100beta
Cellular Localization
Cytoplasm , Nucleus
Background
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX129573 IHC-P Image
S100 beta antibody detects S100 beta protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.S100 beta stained by S100 beta antibody (GTX129573) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX129573 WB Image
S100B antibody detects S100B protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of V5-human S100B-transfected 293T cells
15 % SDS-PAGE
S100B antibody (GTX129573) dilution: 1:1000
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GTX129573 ICC/IF Image
S100 beta antibody detects S100 beta protein at glia cells by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: S100 beta stained by S100 beta antibody (GTX129573) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX129573 IHC-P Image
S100 beta antibody detects S100 beta protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human skin.S100 beta stained by S100 beta antibody (GTX129573) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX129573 IHC-Fr Image
S100 beta antibody detects S100 beta protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: S100 beta stained by S100 beta antibody (GTX129573) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX129573 IHC-P Image
S100B antibody detects S100B protein at glial cell on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
S100B antibody (GTX129573) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX129573 WB Image
Mouse tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody (GTX129573) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129573 IHC-P Image
S100 beta antibody detects S100 beta protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.S100 beta stained by S100 beta antibody (GTX129573) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX129573 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody (GTX129573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129573 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody (GTX129573) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129573 WB Image
The data was published in the journal PeerJ in 2018. PMID: 29888131
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REFERENCE
REVIEW
S100 beta antibody
Cat. No. GTX129573
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Rating
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( Average 4.5 based on 2 users reviews)
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Application
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IHC (Frozen sections)(IHC-Fr)
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( Average 4.5 based on 2 users reviews)
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Date :
Anonymous submitted on 22-Sep-2023
Application Tested :
IHC-Fr
Sample Species :
Rat
Sample :
rat sciatic nerve
Blocking :
5% goat serum in PBS, 37°C, 0.5Hr
Fixation :
4% PFA, 25°C, 16Hr
Primary Antibody :
1:500, 37°C, 1.5Hr
Date :
Anonymous submitted on 09-Nov-2021
Application Tested :
IHC-Fr
Sample Species :
Ms
Sample :
Mouse small intestine tissue
Fixation :
4% PFA in PBS
Permeabilization /Antigen retrieval :
Blocking One Histo (Nacalai Tesque), 10min, 25°C
Primary Antibody :
1:250, 4°C, 16Hr
